CSIR - Centre for Cellular and Molecular Biology

  •   Centre for Cellular & Molecular Biology
    Habsiguda, Uppal Road, Hyderabad
    Telangana - 500007, India
  •  +91 40 27160222-31
  •   Website

Principal Investigator: Dr. K Thangaraj, Dr. Vinay K Nandicoori

Co-Investigators: Dr. Divya Tej Sowpati, Dr. Karthik Bharadwaj Tallapaka

Contributors: Payel Mukherjee, Pratheusa Maccha, Sofia Banu, Sreelekshmi MS, Malini Nemalikanti, Tulasi Nagabandi, Valli Undamatla, Devavrat Desai, Neha Singh, Priya Pandey, G Mala, Deepak Kashyap, Mahfuj Hassan, Vasanth Kumar

Role of the Institution in the GenomeIndia Project:

  • Sample collection and phenotyping from 8 communities
  • Whole genome genotyping and sequencing of 28 communities (~2500 samples) from three different sample collection centres
  • Raw data processing and QC
  • Data analysis at population scale

Accomplishments and Outcomes

  1. Sample collection:

    We have completed sample collection of 1728 from our target communities from Tamil Nadu, Andhra Pradesh, Telangana, and Uttarakhand, including critical tribes. The blood samples from the above were analyzed for various biochemical parameters including CRP, liver function, lipids, lidney function among others. The blood samples were also sent to CBR, Bengaluru for biobanking.

  2. Whole Genome Sequencing:

    Over 3200 samples, including those collected by GBRC and AIIMS-Jodhpur, were genotyped using Illumina GSA, out of which more than 2500 samples were deemed suitable for sequenced. Samples from these communities were sequenced using the Illumina NovaSeq 6000 platform. Raw data was processed to ensure they met the expected quality criteria, and sample wise variants were obtained following the DRAGEN pipeline. Sample level quality checks were performed to remove potential outliers, and those that passed the checks were taken further for population-scale joint genotyping.